Bio Safety Cabinet

“Hygiene Flow Systems” Biosafety cabinets are used as the primary means of containment for working safely with infectious microorganisms. However, biosafety cabinets are only one part of an overall biosafety program, which requires consistent use of good microbiological practices.

Biosafety cabinets are designed to prevent biological exposure to personnel and the environment and may also protect experimental material from being contaminated when appropriate practices and procedures are followed. Three kinds of biosafety cabinets, designated as Class I, II and III have been developed to meet varying research and clinical needs.

Special Features

Construction Material	Powder-coated steel or Stainless Steel (SS 304/316).
Cleanliness Level	ISO Class 5 (Class 100).
Filtration	H14 HEPA filters, 99.99% to 99.9995% efficient at 0.3 µm particles.
Doors	Materials are typically made of tempered safety glass or polycarbonate to ensure durability, visibility, and UV resistance.
Noise Level	Operating noise is generally between 60 ± 5 dBA, with modern designs prioritizing noise reduction for user comfort.
Control Panel	PLC with touchscreen or LED display.
Monitoring	Magnahelic gauge for monitoring pressure drop across filters.
Safety	Emergency stop buttons on Front sides.
Lighting	LED or fluorescent tube lighting.
Options	UV light, Ionizer, or HEPA-filtered ceilings.
Power Supply	230 V AC, Single Phase.

Types of Biosafety Cabinet :

Class I Biosafety Cabinets :

Protection: Personnel and the environment only (no product protection).
How it works: Room air is drawn inward over the work surface, protecting the user from aerosols, before passing through a HEPA filter and being exhausted.
Best for: Protecting the user while handling low-to-moderate risk agents, or enclosing equipment like centrifuges.

Class II Biosafety Cabinets :

Protection: Personnel, product, and the environment.
How it works: An inward airflow protects the user, a HEPA-filtered downward laminar airflow protects the sample, and exhaust air protects the environment. This is the most common type used in laboratories and is subdivided into five categories:
- Type A1: Recirculates 70% of air and exhausts 30%; not recommended for work with volatile toxic chemicals.
- Type A2: The most widely used variant. Similar to A1 but features a higher intake velocity and allows for safe exhaust of small amounts of volatile chemicals if ducted.
- Type B1: Recirculates 30% of air and exhausts 70%; suitable for working with minute quantities of toxic chemicals that are exhausted externally.
- Type B2: “Total exhaust” cabinets. They exhaust 100% of the air out of the building and do not recirculate it. Best for working with toxic chemicals and radionuclides.

Class III Biosafety Cabinets :

Protection: Maximum protection for personnel, product, and the environment.
How it works: A totally enclosed, gas-tight “glove box.” Operations are conducted through heavy-duty attached gloves, and air is supplied and exhausted through HEPA and/or carbon filters.
Best for: High-risk Biosafety Level (BSL) 3 and 4 agents (e.g., exotic agents or those that cause severe/fatal diseases)

Application :

A biological safety cabinet (BSC) is a primary engineering control used to protect personnel against biohazardous or infectious agents and to help maintain quality control of the material being worked with as it filters both the inflow and exhaust air.

Microbiology & Virology
Cell & Tissue Culture
Diagnostic Testing.
Pharmaceutical Labs
Biotechnology

Available Size:

Model: HFB900, HFB1200, HFB1500, HFB1800

Working Size: 3x2x2, 4x2x2, 5x2x2, 6x2x2

Special Features

Types of Biosafety Cabinet :

Application :

Available Size:

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